Photoisomerization of the green fluorescence protein chromophore and the meta- and para-amino analogues
Resource
Chemical Communications 2008: 1344-1346
Journal
Chemical Communications
Journal Issue
11
Pages
1344-1346
Date Issued
2008
Author(s)
Abstract
The Z --> E photoisomerization and fluorescence quantum yields for the wild-type green fluorescence protein (GFP) chromophore (p-HBDI) and its meta- and para-amino analogues (m-ABDI and p-ABDI) in aprotic solvents (hexane, THF, and acetonitrile) and protic solvents (methanol and 10-20% H(2)O in THF) are reported. The dramatic decrease in the quantum yields on going from aprotic to protic solvents indicates the important role of solvent-solute hydrogen bonding in the nonradiative decay pathways. The enhanced fluorescence of m-ABDI is also discussed.
Type
journal article
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