Study of the oral administration strategies with Schizophyllan in Tilapia non-specificity immune factor
Date Issued
2011
Date
2011
Author(s)
Juan, Yun-Ting
Abstract
The purpose of this study to know the effect of Schizophyllan is extracted from the culture medium of Schizophyllum Commune on non-specific immune response of Tilapia by oral administration. The preparation different concentrations of polysaccharide solution and with give different number of observations of different Tilapia non-specific immune effects. Daily observe of non-specific detection of immune factors, including the superoxide anion production, nitric oxide production and lysozyme activity.
The results found in (1) to give the same number but different concentrations of polysaccharide solution, given once and twice time experimental group to the concentration of 500ppm and 250ppm in the dose can be better improved results and sustained effect is better. Giving four times experimental groups given low doses of 62.5ppm places concentration was better, sustained results are better. (2) the same total dose of polysaccharide solution, give the times of different circumstances. Superoxide anion production in the medium-dose is given twice into the ability to enhance a better and sustained effect also better than other. Nitric oxide production in the high-dose is given once to enhance the ability to better circumstances. Lysozyme activity in the high-dose and medium-dose given in the previous days has improved significantly, four times given low doses significantly improved in the latter. (3) the same concentration of polysaccharide solution, given the number of different circumstances, superoxide anion production and the nitric oxide production to give twice group are improved to give better results, and the continuing validity longer. Lysozyme activity in the high-dose given once and twice given the circumstances have improved in the early phenomenon, giving four times, in the late enhance the effect.
Continuing validity of the whole, the results show that give the four times groups low doses of 62.5ppm best, both in the superoxide anion production, nitric oxide production and lysozyme activity has improved significantly, and with other groups another comparison, the continued effectiveness is better. Therefore, by inference results given tilapia β-glucan can be used to enhance immunity to low doses of 62.5ppm given four times and stop until 12 days give again the feeding strategy.
Subjects
Tilapia
Schizophyllan
non-specificity immune
Type
thesis
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