Novel transplant of combined platelet-rich fibrin Releasate and bone marrow stem cells prevent bone loss in Ovariectomized osteoporotic mice
Journal
BMC Musculoskeletal Disorders
Journal Volume
21
Journal Issue
1
Date Issued
2020
Author(s)
Abstract
Background: Osteoporosis is a metabolic bone disorder characterized by deterioration in the quantity and quality of bone tissue, with a consequent increase susceptibility to fracture. Methods: In this study, we sought to determine the efficacy of platelet-rich fibrin releasates (PRFr) in augmenting the therapeutic effects of stem cell-based therapy in treating osteoporotic bone disorder. An osteoporosis mouse model was established through bilateral ovariectomy on 12-week-old female ICR (Institute of Cancer Research) mice. Eight weeks postoperatively, the ovariectomized (OVX) mice were left untreated (control) or injected with PRFr, bone marrow stem cells (BMSCs), or the combination of BMSCs and PRFr. Two different injection (single versus quadruple) dosages were tested to investigate the accumulative effects of BMSCS and PRFr on bone quality. Eight weeks after injection, the changes in tibial microstructural profiles included the percentage of bone volume versus total tissue volume (BV/TV, %), bone mineral density (BMD, g/cm3), trabecular number (Tb.N, number/mm), and trabecular separation (Tb.Sp, mm) and bony histology were analyzed. Results: Postmenopausal osteoporosis model was successfully established in OVX mice, evidenced by reduced BMD, decreased BV/TV, lower Tb.N but increased Tb.Sp. Eight weeks after injection, there was no significant change to BMD and bone trabeculae could be detected in mice that received single-injection regimen. In contrast, in mice which received 4 doses of combined PRFr and BMSCs, the BMD, BV/TV, and TB.N increased, and the TB.Sp decreased significantly compared to untreated OVX mice. Moreover, the histological analysis showed the trabecular spacing become narrower in OVX-mice treated with quadruple injection of BMSCs and combined PRFr and BMSCs than untreated control. Conclusion: The systemic administration of combined BMSCs and PRFr protected against OVX-induced bone mass loss in mice. Moreover, the improvement of bony profile scores in quadruple-injection group is better than the single-injection group, probably through the increase in effect size of cells and growth factors. Our data also revealed the combination therapy of BMSCs and PRFr has better effect in enhancing osteogenesis, which may provide insight for the development of a novel therapeutic strategy in osteoporosis treatment. ? 2020 The Author(s).
Subjects
growth factor; platelet-rich fibrin; platelet-rich fibrin; animal cell; animal experiment; animal model; animal tissue; Article; bone cell; bone density; bone development; bone mass; bone quality; bone structure; bone tissue; bone volume; bone volume fraction; controlled study; drug synthesis; effect size; female; hematopoietic stem cell transplantation; micro-computed tomography; mouse; nonhuman; osteolysis; outcome assessment; ovariectomy-induced osteoporosis; postmenopause osteoporosis; postoperative period; single drug dose; systemic therapy; therapy effect; tibia; trabecular bone; trabecular number; treatment response; animal; bone marrow cell; human; Institute for Cancer Research mouse; osteoporosis; ovariectomy; Animals; Bone Density; Bone Marrow Cells; Female; Humans; Mice; Mice, Inbred ICR; Osteoporosis; Ovariectomy; Platelet-Rich Fibrin
SDGs
Other Subjects
growth factor; platelet-rich fibrin; platelet-rich fibrin; animal cell; animal experiment; animal model; animal tissue; Article; bone cell; bone density; bone development; bone mass; bone quality; bone structure; bone tissue; bone volume; bone volume fraction; controlled study; drug synthesis; effect size; female; hematopoietic stem cell transplantation; micro-computed tomography; mouse; nonhuman; osteolysis; outcome assessment; ovariectomy-induced osteoporosis; postmenopause osteoporosis; postoperative period; single drug dose; systemic therapy; therapy effect; tibia; trabecular bone; trabecular number; treatment response; animal; bone marrow cell; human; Institute for Cancer Research mouse; osteoporosis; ovariectomy; Animals; Bone Density; Bone Marrow Cells; Female; Humans; Mice; Mice, Inbred ICR; Osteoporosis; Ovariectomy; Platelet-Rich Fibrin
Type
journal article