Development and biochemical characterization of the monoclonal antibodies for specific detection of the emerging H5N8 and H5Nx avian influenza virus hemagglutinins
Journal
Applied Microbiology and Biotechnology
Journal Volume
105
Journal Issue
1
Pages
235-245
Date Issued
2021
Author(s)
Abstract
Abstract: The highly pathogenic avian influenza (HPAI) H5N8 virus has been detected in wild birds and poultry worldwide. The threat caused by HPAI H5N8 virus still exists with concerns for human infection. The preparedness for epidemic prevention and decreasing the agricultural and economic lost is extremely important. Hemagglutinin (HA), a surface glycoprotein of influenza viruses, is considered as the major target for detection of the influenza virus subtype in the infected samples. In this study, the recombinant H5N8 HA1 and HA2 proteins were expressed in Escherichia coli, and were utilized to generate two monoclonal antibodies, named 7H6C and YC8. 7H6C can bind the HA proteins of H5N1 and H5N8, but cannot bind the HA proteins of H1N1, H3N2, and H7N9, indicating that it has H5-subtype specificity. In contrast, YC8 can bind the HA proteins of H1N1, H5N1, and H5N8, but cannot bind the HA proteins of H3N2 and H7N9, indicating that it has H1-subtype and H5-subtype specificity. The epitope sequences recognized by 7H6C are located in the head domain of H5N8 HA, and are highly conserved in H5 subtypes. The epitope sequences recognized by YC8 are located in the stalk domain of H5N8 HA, and are highly conserved among the H1 and H5 subtypes. 7H6C and YC8 can be applied for specific detection of the HA proteins of H5N8 and H5Nx avian influenza viruses. Key points: ? The mAb 7H6C or YC8 was generated by using the HA1 or HA2 of the HPAI H5N8 virus as the immunogen. ? 7H6C recognized the head domain of H5N8 HA, and YC8 recognized the stalk domain of H5N8 HA. ? 7H6C and YC8 can detect the HA proteins of H5Nx subtypes specifically. ? 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
Subjects
Agricultural robots; Chemical detection; Epitopes; Escherichia coli; Monoclonal antibodies; Viruses; Avian influenza virus; Biochemical characterization; Highly pathogenic avian influenzas; Human infection; Influenza virus; Specific detection; Surface glycoproteins; Wild birds; Recombinant proteins; epitope; Influenza virus hemagglutinin; Influenza virus hemagglutinin ha1; Influenza virus hemagglutinin ha2; monoclonal antibody; monoclonal antibody 7h6c; monoclonal antiboy yc8; unclassified drug; hemagglutinin; Influenza virus hemagglutinin; monoclonal antibody; antibody; avian influenza; biochemistry; detection method; epidemic; infectious disease; influenza; poultry; protein; virus; Article; binding affinity; enzyme linked immunosorbent assay; epitope mapping; hemagglutination; hemagglutination inhibition; Influenza A virus (H1N1); Influenza A virus (H3N2); Influenza A virus (H5N1); Influenza A virus (H5N8); Influenza A virus (H7N9); nonhuman; animal; avian influenza; genetics; human; Influenza A virus (H1N1); Influenza A virus (H5N1); Influenza A virus (H7N9); Avian influenza virus; Escherichia coli; H3N2 subtype; H7N9 subtype; Influenza A virus; Orthomyxoviridae; Animals; Antibodies, Monoclonal; Hemagglutinin Glycoproteins, Influenza Virus; Hemagglutinins; Humans; Influenza A Virus, H1N1 Subtype; Influenza A Virus, H3N2 Subtype; Influenza A Virus, H5N1 Subtype; Influenza A Virus, H7N9 Subtype; Influenza in Birds
SDGs
Other Subjects
Agricultural robots; Chemical detection; Epitopes; Escherichia coli; Monoclonal antibodies; Viruses; Avian influenza virus; Biochemical characterization; Highly pathogenic avian influenzas; Human infection; Influenza virus; Specific detection; Surface gly
Type
journal article