光生化型晶片系統於藥物篩選與疾病檢測之研發－ 特用型光生化型晶片儀(子計畫一)
Date Issued
2005-07-31
Date
2005-07-31
Author(s)
DOI
932323B002012
Abstract
Human beings have been threatened by tuberculosis for a long time. However, as the
mortality rising gradually, especially those from depressed area, this problem is getting more
seriously as time goes by. Death count that can be attributed to tuberculosis every year is more
than the summation of those for AIDS, malaria, diarrhea and tropical diseases. In addition to
x-ray photograph and test of skin tubercle bacillus, sputum test is currently the major way to
diagnose tuberculosis. This method includes smear dyeing of mycobacteria and germiculture.
However, sputum test suffers from low sensitivity and long detection time. Besides, repeatedly
test, it usually takes more than a month to get a complete report. The opportune moment is
often wasted. While more the more people suspect the effect of BCG vaccine, it is necessary to
develop new methods to handle large number of tubercle bacillus detection. The vigorously
progressing biochip is a promising technique.
The trends of biochip-related technique are as follows. (1) Use non-contact technique to
avoid influence the sample. (2) To achieve high sensitivity, wide bandwidth and small probe
volume. At the same time as the worldwide trend to reduce of the sample for biochip,
opto-electric technique is the most suitable detection method. Out of all these opto-electronic
techniques, surface plasmon resonance, ellispsometry, and waveguide interferometry developed
within the last year are some of the most promising techniques for wide spread use.
This sub-project is in charge of development of the TBMorph (Tuberculosis Morph) in
coordination with the main project to pursue the research on the “Opto-BioMorphin” that used in
the epidemic detection and the drug development. On one hand, the phase detection surface
plasmon resonance has been brought up and been integrated into TBMorph. The measuring
speed of Ellipsometer function has been improved and the speed has increased 10 times. This
became a new advantage for TBMorph’s applications. On the other hand, there is a very
important breakthrough of this year execution. The rotating analyzer-based dual polarized
waveguide interferometer has been successfully accomplished. This newly developed technique
not only adopts Waveguide Interferometry but also integrates the dual polarized phase shifting
metrology. The system thus has the potential to have the real-time measurement ability.
Besides the development of the detecting platforms, the preliminary results of biochemical
binding reaction between TB and antibody were also carried out by TBMorph in this sub-project.
It proved that TBMorph has the ability to select the better antibody that has strong specificity to
pulmonary tuberculosis. The newly developed technique, “the rotating analyzer-based dual
polarized waveguide interferometer”, also has been proved its ability in bio-reactions
measurement. The reaction between human IgG and anti-IgG has been successfully measured.
Moreover, TBMorph can also detect the variation in reflection index due to the change in the
concentration of glucose by utilizing phase detection SPR function. The experimental results of
glucose detection are a strong support that TBMorph can be widely used in biological
examination.
Subjects
Opto-BioMorphin
OBMorph
biochip
ellipsometer
Surface Plasmon Resonance
Waveguide Interferometry
Publisher
臺北市：國立臺灣大學應用力學研究所
Type
report
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