Submergence gene sub1a transfer into drought-tolerant japonica rice dt3 using marker-assisted selection
Journal
International Journal of Molecular Sciences
Journal Volume
22
Journal Issue
24
Date Issued
2021
Author(s)
Abstract
Flash flooding is a major environmental stressor affecting rice production worldwide. DT3 is a drought-tolerant, recurrent parent with a good yield, edible quality, and agronomic traits akin to those of an elite Taiwanese variety, Taiken9 (TK9). Progenies carrying Sub1A can enhance submergence stress tolerance and can be selected using the marker-assisted backcross (MAB) breeding method. For foreground selection, Sub1A and SubAB1 were utilized as markers on the BC2 F1, BC3 F1, and BC3 F2 generations to select the submergence-tolerant gene, Sub1A. Background selection was performed in the Sub1A-BC3 F2 genotypes, and the percentages of recurrent parent recovery within individuals ranged from 84.7–99.55%. BC3 F3 genotypes (N = 100) were evaluated for agronomic traits, yield, and eating quality. Four of the eleven BC3 F4 lines showed good yield, yield component, grain, and eating quality. Four BC3 F4 lines, SU39, SU40, SU89, and SU92, exhibited desirable agronomic traits, including grain quality and palatability, consistent with those of DT3. These genotypes displayed a high survival rate between 92 and 96%, much better compared with DT3 with 64%, and demonstrated better drought tolerance compared to IR64 and IR96321-345-240. This study provides an efficient and precise MAB strategy for developing climate-resilient rice varieties with good grain quality for flood-prone regions. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Subjects
Background selection
Drought tolerance
Foreground selection
Japonica DT3
Marker-assisted backcross
Submergence tolerance
adult
agronomic trait
article
background selection
climate
controlled study
drought tolerance
eating
female
genetic marker
genotype
human
Japonica rice
major clinical study
male
nonhuman
palatability
remission
survival rate
adaptation
drought
flooding
genetics
metabolism
Oryza
phenotype
physiological stress
plant breeding
plant gene
procedures
plant protein
Adaptation, Physiological
Droughts
Floods
Genes, Plant
Genotype
Phenotype
Plant Breeding
Plant Proteins
Stress, Physiological
Type
journal article