Elevated serum levels of mucin-associated antigen in patients with acute respiratory distress syndrome
Journal
American Journal of Respiratory and Critical Care Medicine
Journal Volume
156
Journal Issue
5
Pages
1453-1457
Date Issued
1997
Author(s)
Abstract
Increased serum levels of mucin-associated antigen have been previously demonstrated in patients with cystic fibrosis (CF) and interstitial pneumonia, and in lung-transplant recipients. The present study assessed the serum airway mucin levels in patients with acute respiratory distress syndrome (ARDS). An enzyme-linked immunosorbent assay (ELISA) method with a human-airway-mucin-specific monoclonal antibody (17Q2) was used to measure serum mucin levels in normal subjects, chronic smokers, patients with chronic bronchitis and other pulmonary diseases, patients with acute cardiogenic lung edema, and patients with ARDS. The serum mucin levels measured 9.9 ± 0.8 ng/ml (mean ± SEM, n = 59) in normal subjects, 12.7 ± 1.6 ng/ml (n = 29) in chronic smokers, 21.8 ± 1.9 ng/ml (n = 28) in patients with chronic bronchitis and other pulmonary diseases, 9.0 ± 3.1 ng/ml (n = 5) in patients with acute cardiogenic lung edema. The serum mucin level was 53.8 ± 6.6 ng/ml (n = 13) in patients with ARDS (p < 0.05, as compared with the four other groups). Serial measurements of serum mucin levels were obtained in patients with ARDS. Statistical analysis showed an inverse correlation of serial measurements of serum mucin with static respiratory-system compliance (p = 0.021), an inverse correlation of sequential serum mucin levels and log(Pao2/Fio2) (p = 0.016), and a positive correlation of sequential serum mucin levels and lung injury score (LIS) (p = 0.019). Gel-filtration analysis showed that mucin-associated antigens in ARDS sera were polydispersed and smaller than the antigens in normal sera. This study indicates that an increasing amount of degraded mucin occurs in patients with ARDS.
SDGs
Other Subjects
antigen; mucin; adult; adult respiratory distress syndrome; aged; article; chronic bronchitis; clinical article; controlled study; enzyme linked immunosorbent assay; female; gel filtration chromatography; human; lung compliance; lung edema; male; priority journal; respiratory distress syndrome; smoking
Publisher
American Thoracic Society
Type
journal article