Identification of soluble proteins and corresponding genes from early developing anther that differentiate normal and heat-tolerant tomato lines
Date Issued
2006
Date
2006
Author(s)
Lu, Hung-Chou
DOI
zh-TW
Abstract
According to previous study, developing anther 7-15 days before anthesis in tomatoes was the most sensitive period to high temperature which causes a low fruit set. In order to identify the proteins which are responsible for the better fruit set in heat-tolerant variety than in heat-sensitive one, we used two dimensional electrophoresis systems to separate isolated proteins from developing anther and looked for the proteins up-/down-regulated in heat-tolerant variety. In doing so, two selected cultivars, CL5915, the heat-tolerant, and L4783, the heat-sensitive, were grown at the same time in a phytotron at both 30/25℃ and 25/20℃. The proteins isolated from developing anthers of heat-tolerant variety grown in 30/25℃, were subtracted to remove the common genetic background. The resulting populations of proteins were considered as induced protein positive to heat stress. Using LC-ESI MS/MS, several proteins were identified, including glyoxalase I and 3beta-hydroxysteriod dehydrogenase. Degenerative primers were designed and RACE technique was applied to obtain the complete gene sequences of the two-mentioned proteins from heat-sensitive and heat-tolerant tomatoes. I found substitution of nucleotides of both proteins can explain accurately the changes in isoelectric point and molecular weight of these two proteins.
Subjects
耐熱
番茄
雄蕊
二維電泳
heat-tolerant
tomato
anther
two dimensional electrophoresis
Type
other
File(s)![Thumbnail Image]()
Loading...
Name
ntu-95-R93b42021-1.pdf
Size
23.31 KB
Format
Adobe PDF
Checksum
(MD5):f2d96eab590a1f2a793281f361d67256