Sex identification in the Collared Scops Owl (Otus bakkamoena) with novel markers generated by random amplified polymorphic DNA
Journal
Conservation Genetics Resources
Journal Volume
5
Journal Issue
1
Pages
239-242
Date Issued
2013
Author(s)
Hsu H.A.
Chao M.C.
Chan F.T.
Wang L.M.
Lin P.I.
Tsao H.S.
Chen C.C.
Abstract
The Collared Scops Owl (Otus bakkamoena) is not sexually dimorphic, which makes it difficult to discriminate between sexes. Hence, it is difficult to establish a breeding and conservation program. This study report, the identification of a novel random amplified polymorphic DNA (RAPD) marker for sexing the species O. bakkamoena. A novel 1,307-bp sex-specific fragment, AN15, in females was initially generated by RAPD fingerprinting using the primer OPAN15. We then designed the nested primer set, CsoAN15-6F/R, and amplified a sex-specific fragment of 349 bp in females, as well as a 796-bp fragment shared by both sexes. The latter fragment can serve as an internal control for monitoring both the PCR reaction and preventing false sex assignment. Our current study accurately identified the sex of each individual (including 8 males and 8 females) using this primer set. This novel primer set, thus, provides a sex-specific DNA marker for accurate sex identification in this species. © 2012 Springer Science+Business Media Dordrecht.
SDGs
Type
journal article