Characterization of medium-chain-length polyhydroxyalkanoate biosynthesis by Pseudomonas mosselii TO7 using crude glycerol
Journal
Bioscience, Biotechnology and Biochemistry
Journal Volume
82
Journal Volume
82
Journal Issue
3
Journal Issue
3
Pages
532-539
Start Page
532
End Page
539
ISSN
09168451
Date Issued
2018
Author(s)
Abstract
Polyhydroxyalkanoates (PHAs) are biopolyesters produced by microorganisms that are environmentally friendly. PHAs can be used to replace traditional plastic to reduce environmental pollution in various fields. PHA production costs are high because PHA must be produced from a carbon substrate. The purpose of this study was to find the strain that can used the BDF byproduct as the sole carbon source to produce high amounts of medium-chain-length PHA. Three isolates were evaluated for potential PHA production by using biodiesel-derived crude glycerol as the sole carbon source. Among them, Pseudomonas mosselii TO7 yielded high PHA content. The PHA produced from P. mosselii TO7 were medium-chain-length-PHAs. The PHA content of 48% cell dry weight in 48 h with a maximum PHA productivity of 13.16 mg PHAs L-1 h-1. The narrow polydispersity index value of 1.3 reflected the homogeneity of the polymer chain, which was conducive to industrial applications.
Subjects
Biodiesel fuel
Biosynthesis
Crude glycerol
Polyhydroxyalkanoate
Pseudomonas mosselii TO 7
Other Subjects
Bacteria; Biochemistry; Biodiesel; Biosynthesis; Glycerol; Substrates; Bio-diesel fuel; Crude glycerol; Environmental pollutions; Medium-chain length PHA; Poly-hydroxyalkanoate; Polydispersity indices; Polyhydroxyalkanoates; Pseudomonas mosselii TO 7; Chain length; glycerol; polyhydroxyalkanoic acid; biosynthesis; chemistry; metabolism; Pseudomonas; Glycerol; Polyhydroxyalkanoates; Pseudomonas
Publisher
Japan Society for Bioscience Biotechnology and Agrochemistry
Type
journal article