Application of CHD1 Gene and EE0.6 Sequences to Identify Sexes of Several Protected Bird Species in Taiwan
Resource
Taiwania, 56(2), 105-110
Journal
Taiwania
Journal Volume
56
Journal Issue
2
Pages
105-110
Date Issued
2011-06
Date
2011-06
Abstract
Many bird species, for example: Crested Serpent Eagle (Spilornis cheela hoya), Collared Scops (Owl Otus bakkamoena), Tawny Fish Owl (Ketupa flavipes), Crested Goshawk (Accipiter trivirgatus), and Grass Owl (Tyto longimembris)... etc, are monomorphic, which is difficult to identify their sex simply by their outward appearance. Especially for those monomorphic endangered species, finding an effective tool to identify their sex beside outward appearance is needed for further captive breeding programs or other conservation plans. In this study, we collected samples of Black Swan (Cygmus atratus) and Nicobar Pigeon (Caloenas nicobarica), two aviaries introduced monomorphic species served as control group, and Crested Serpent Eagle, Collared Scops Owl, Tawny Fish Owl, Crested Goshawk, and Grass Owl, five protected monomorphic species in Taiwan. We used sex-specific primers of avian CHD1 (chromo-helicase-DNA-binding) gene and EE0.6 (EcoRI 0.6-kb fragment) sequences to identify the sex of these birds. The results showed that CHD1 gene primers could be used to correctly identify the sex of Black Swans, Nicobar Pigeons and Crested Serpent Eagles, but it could not be used to correctly identify sex in Collared Scops Owls, Tawny Fish Owls, and Crested Goshawks. In the sex identification using EE0.6 sequence fragment, A, C, D and E primer sets could be used for sexing Black Swans; A, B, C, and D primer sets could be used for sexing Crested Serpent Eagles; and E primer set could be used for sexing Nicobar Pigeons and the two owl species. Correct determination of sex is the first step if a captive breeding measure is required. We have demonstrated that several of the existing primer sets can be used for sex determination of several captive breeding and indigenous bird species.
Subjects
領角鴞、大冠鷲、鳳頭蒼鷹、鳥類性別鑑定、黃魚鴞
Collared Scops Owl, Crested Serpent Eagle, Crested Goshawk, sex identification of bird, Tawny Fish Owl
Type
journal article
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