Enhancing the antitumor cell proliferation and Cu2+-chelating effects of black soybeans through fermentation with aspergillus awamori
Journal
Journal of Bioscience and Bioengineering
Journal Volume
115
Journal Issue
4
Pages
400-404
Date Issued
2013
Author(s)
Abstract
In the present study, black soybeans were fermented with Aspergillus awamori at 30¢XC for 3 days. The effect of fermentation on the antiproliferative effect against human colon cancer cells, Caco-2 and HT-29 as well as Cu2+-chelating effect of black soybeans was investigated. It was found that the water, 80% methanol or 80% ethanol extract of fermented black soybeans showed a significantly higher (P < 0.05) antiproliferative and Cu2+-chelating effect than did the respective extract of non-fermented black soybeans. Generally, the methanol extract and the ethanol extract of fermented black soybeans exerted higher antiproliferative effect on both Caco-2 and HT-29 cells. While water extract of fermented black soybeans showed the highest Cu2+-chelating effect among the various extracts examined. Taking into account of extraction yields further revealed that bioactive principles that exhibit Cu2+-chelating effect could be extracted to the largest extent with water as the extraction solvent. With same amount of sample, water extract obtained from fermented black soybeans possesses the highest Cu2+-chelating abilities. ? 2012 The Society for Biotechnology, Japan.
Subjects
Antiproliferative effect
Aspergillus awamori
Black soybean
Cu2+-chelating ability
Fermented
SDGs
Other Subjects
Antiproliferative effect; Aspergillus awamori; Black soybean; Chelating ability; Fermented; Aspergillus; Cell proliferation; Chelation; Ethanol; Fermentation; Methanol; Extraction; alcohol; antineoplastic agent; black soybean extract; copper; methanol; plant extract; soybean protein; unclassified drug; water; antineoplastic agent; chelating agent; copper; plant extract; antineoplastic activity; antiproliferative activity; article; Aspergillus awamori; black soybean; cancer cell; cell proliferation; chelation; colon cancer; controlled study; drug isolation; fermentation; human; human cell; nonhuman; soybean; Aspergillus; CACO 2 cell line; drug effects; HT 29 cell line; metabolism; microbiology; physiology; soybean; Aspergillus awamori; Glycine max; Antineoplastic Agents, Phytogenic; Aspergillus; Caco-2 Cells; Cell Proliferation; Chelating Agents; Copper; Fermentation; HT29 Cells; Humans; Plant Extracts; Soybeans
Type
journal article