Combination of Bone Marrow Derived Stem Cells and Dental Pulp Derived Stem Cells with Platelet-rich Fibrin Releasate (PRFr) to Repair and Regenerate Sciatic Nerves Injury

The injury of sciatic nerves was a serious problem to the patients, such as costing money for treatment and even worse to change their life. However, there were many limitations in the traditional therapy and as a result a better treatment should be found out. Recently, the cell-based therapy has been focused to repair the broken-down nerves. In the mean times, using Platelet-rich fibrin releasate (PRFr) to repair damaged organs was an insight in Regenerative Medicine. Bone marrow derived stem cells (BMSCs) and Dental pulp derived stem cells (DPSCs) and PRFr were combined to treat with the animals. The 8 week old- injured nerve rats were separated into 6 groups. (1.) Control group, without any treatment; (2.) PRFr group,0.1 ml PRFr was injected; (3.) BMSCs group, 1x106 / 0.1 ml BMSCs were injected; (4.) DPSCs group, 1x106 / 0.1 ml DPSCs were injected; (5.) BMSC and PRFr group; (6.) DPSC and PRFr group, the rats were treated with 0.1 ml BMSCs and PRFr. Twelve weeks later, rats were sacrificed and the sciatic nerves regeneration was evaluated by gross and microscope. In gross, we measured the diameters of the regeneration sciatic nerves of every rats, then average every group and compared each other; in microscope, we calculate the axon density in the regeneration part of nerve fiber and average every group and compared. The result demonstrated that the combined treatment has significant impact than other groups. Following that was stem cells (BMSCs, DPSCs) treatment. However, the PRFr group was not efficiency as our expect but it still reached significant than the control group. In conclusion, cell based therapy may provide a new way to the nerve regeneration.