The Stability for Cytoplasmic Male Sterile in Pepper
Date Issued
2009
Date
2009
Author(s)
Yin, Miao-Jen
Abstract
Abstract Cytoplasmic male sterility (CMS) is the result of interaction between cytoplasm and nuclear genes. The sterility of the CMS pepper lines was not always stable. Some of them might restore their fertility partially or completely due to low temperature, and such restoration could be resumed as temperature re-elevate. The objective of this thesis is to study the genetic background of collected pepper lines, the influences of temperature and seasonal changes on CMS pepper lines, and the anatomy difference between stable and unstable CMS line. All CMS pepper lines could generate predicted a (S)ψatp6-2 fragment by primer sets, while their maintainer lines couldn’t produce the predicted products, and similar results were obtained for orf456 fragment. These results revealed that the cytoplasms of all the CMS pepper lines contained (S)ψatp6-2 and orf456, and their maintainers of hot pepper lines, with N cytoplasm, didn’t have such defectedψatp6-2 and orf456. Surprisingly, ‘PBC 84 selex’ and ‘9946-2138’, maintainer lines of unstable CMS sweet pepper, could generate orf456 fragment by CMS P1 or CMS P2 primer sets, representing that the genetic background of cytoplasm in specific maintainers might be more complicated and different from other maintainers. Unstable CMS hot pepper line ‘CCA 7243’ could restore fertility in the greenhouse and in the field while the night temperature was below 21 oC, and also could restore in the 20/15 oC phytotron. Plants of unstable CMS sweet pepper line ‘CCA 7236’ restored fertility while night temperature was below 21oC or 17 oC, but plants hardly restored while day temperature below 18 oC. Besides, the fertility restoration of unstable CMS lines showed as a regular cycle, not always steady expressed. When night temperature went higher, both lines were reverted male sterile. Both CMS line ‘CCA 7244’ and ‘CCA 7234’ almost expressed male sterile even under a long period of lower night temperature. As unstable CMS line ‘CCA7243’ expressed sterility under high temperature, abnormalities in tapetum cells were very different from the results of stable CMS line ‘CCA7244’. While tetrads formed, locule in ‘CCA 7243’ may or may not increase in size as compared with locular space did not increase in ‘CCA 7244’. As microspores development, the appearance of tapetum in ‘CCA 7243’ with dense cytoplasm or containing a vacuole was different from vacuolated tapetum in ‘CCA 7244’, and timing of tapetum cells degeneration was hardly identified, but tapetum cells in both lines were gone at the end. These anatomical data revealed that the genetic control might be more complicated in unstable CMS line. As the anthers of ‘CCA7243’ resumed the fertility, there was no difference between ‘CCA7243’ and its maintainer ‘PBC 385’ anatomically.
Subjects
anther differentiation
Capsicum annuum L.
cytoplasmic male sterility (CMS)
microgametogenesis
temperature
Type
thesis
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