Corylin reduces obesity and insulin resistance and promotes adipose tissue browning through SIRT-1 and β3-AR activation
Journal
Pharmacological research
Journal Volume
164
Date Issued
2021-02
Author(s)
Abstract
Brown adipose tissue (BAT) activation or beige adipocytes in white adipocytes (WAT) (browning) is a novel strategy against obesity. Corylin, a flavonoid compound extract from Psoralea corylifolia L., has been shown to exert anti-inflammatory, anticancer, and anti-atherosclerotic effects and ameliorate hyperlipidemia and insulin resistance. However, the therapeutic effect of corylin on obesity remains unknown. The objective of this study was to evaluate the effect of corylin on browning or obesity. Here, we report that corylin induced browning by elevating the expression levels of beige- or browning-specific marker genes, including cited1, hoxc9, pgc1α, prdm16, and ucp1, in 3T3-L1 adipocytes, WAT and BAT. Moreover, corylin also strikingly reduced body weight and fat accumulation and increased insulin sensitivity, mitochondrial biogenesis, and β-oxidation in HFD- and DIO-treated mice. The browning and lipolysis effects of corylin were abolished by sirtuin 1 (SIRT1) inhibitor (EX527) and β3-adrenergic receptor (β3-AR) antagonist (L-748,337) treatment. The possible molecular mechanism of corylin on the browning and lipolysis of adipocytes is through SIRT1- or β3-AR-dependent pathways. The study suggested that corylin exerts anti-obesity effects through the browning of white adipocytes, activating of BAT and promoting of lipid metabolism. Therefore, corylin may be a helpful therapeutic candidate for treating obesity.
Subjects
Browning; Corylin; Obesity; SIRT1; UCP1; β3-AR
SDGs
Other Subjects
beta 3 adrenergic receptor; corylin; flavonoid; sirtuin 1; unclassified drug; antiobesity agent; beta 3 adrenergic receptor; corylin; flavonoid; Sirt1 protein, mouse; sirtuin 1; 3T3-L1 cell line; animal cell; animal experiment; animal model; animal tissue; antiobesity activity; Article; beige adipocyte; body weight loss; brown adipose tissue; cell activation; cited1 gene; controlled study; drug effect; drug mechanism; energy expenditure; fatty acid oxidation; gene; gene expression; hoxc9 gene; insulin resistance; insulin sensitivity; lipid metabolism; lipid storage; lipolysis; male; mitochondrial biogenesis; mouse; nonhuman; obesity; pgc1alpha gene; prdm16 gene; priority journal; protein expression; signal transduction; ucp1 gene; white adipocyte; adipocyte; animal; C57BL mouse; insulin resistance; lipid diet; metabolism; obesity; white adipose tissue; 3T3-L1 Cells; Adipocytes; Adipose Tissue, Brown; Adipose Tissue, White; Animals; Anti-Obesity Agents; Diet, High-Fat; Flavonoids; Insulin Resistance; Lipolysis; Male; Mice; Mice, Inbred C57BL; Obesity; Receptors, Adrenergic, beta-3; Sirtuin 1
Type
journal article