行政院國家科學委員會三年期專題研究計畫之第二年成果報告計畫:Salmonella choleraesuis之鐵調節基因與毒力表現（2/3)
Date Issued
2001
Date
2001
Author(s)
張照夫
DOI
892313B002141
Abstract
To identify genes belonging to the Fur regulon of Salmonella enterica serovar Choleraesuis, the Fur titration assay (FURTA) was used to screen a genomic library for Fur promoters and iron-regulated genes. Fifteen FURTA-positive clones were identified and DNA-sequence analysis showed that eleven bad a Fur-binding site (Fur box), and six showed homology to the iron-regulated genes of S. enterica serovar Typhi and/or K coil. One of these clones (pSC4) showed homology to the/roB gene of the imA locus of S. enfrrica serovar Typhi. The iroA Locus of S. enter/ca serovar Choleraesuis was cloned from a X-dash library and subjected to DNA sequencing. The complete nucleotide sequence of 9,848 bp of the irnA locus of S enterica serovar Choleraesuis, which consists of 1mB, C, 13, E, and N genes and was transcriptionally regulated by Fur, was 97% identical to that of ¢G enterica serovar Typhi. The iroN showed homology to the family of TonB-dependent outer membrane receptors and a putative virulence factor, iroNa,g, of the extraintestinal pathogen E. coil. The convalescent porcine sera contained antibodies against the three major iron-regulated outer membrane proteins of S enterica serovar Choleraesuis. An insertional inactivation of the lioN gene of S enterica serovar Choleraesuis by allelic exchange resulted in the loss of expression of the 78- Da protein. However, this mutant had an LD50 for mice similar to that of the parent strain when administered by the intraperitoneal route.
Subjects
豬溫沙氏桿菌
攝鐵基因
FURTA
對偶基因交換技術
iroN突變株
SDGs
Type
report
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