Red mold rice promotes neuroprotective sappalpha secretion instead of alzheimer's risk factors and amyloid beta expression in hyperlipidemic aβ40-lnfused rats
Journal
Journal of Agricultural and Food Chemistry
Journal Volume
58
Journal Issue
4
Pages
2230-2238
Date Issued
2010
Author(s)
Abstract
Amyloid beta (Aβ) peptide is closely related to the onset of Alzheimer's disease (AD). A high-cholesterol or high-energy diet was demonstrated to stimulate Aβ formation and deposition in the amyloid precursor protein (APP) pathway and, oppositely, downregulate the secretion of the neuroprotective soluble APP α-fragment (sAPPα). Wonascus-fermented red mold rice (RMR) Including multiple cholesterol-lowering agents, antioxidants, and anti-inflammatory agents has been proven to ameliorate Aβ40 infusion-Induced memory deficit in our previous study. In this study, the ethanol extract of RMR (RE) and natural RMR were respectively tested for their effect on the mediation of the proteolytic process of APP in cholesterol-treated human neuroblastoma IMR32 cell, as well as their effect on memory and learning ability and the expression of AD risk factors in intracerebroventricular Aβ40-infused hyperlipidemic rats. In the results, RE suppressed cholesterolraised -secretase activity and further resulted in the increase of sAPPα secretion in the IMR32 cell. In the animal test, RMR potently reversed the memory deficit in the water maze and passive avoidance tasks. RMR administration could prevent against Aβ40 infusion plus the great damage caused by a high energy diet in hippocampus and cortex involved in the raise of thiobarbituric acid reactive substances and reactive oxygen species. The neuroprotection provided by RMR downregulates Aβ40 formation and deposition by suppressing the cholesterol-raised β-secretase activity and apollpoprotein E expression, as well as mediates the proteolytic process of APP toward neuroprotective sAPPα secretion in hippocampus. ?2010 American Chemical Society.
SDGs
Other Subjects
amyloid beta protein; amyloid beta protein[1-40]; cholesterol; mevinolin; peptide fragment; reactive oxygen metabolite; secretase; thiobarbituric acid reactive substance; Alzheimer disease; animal; article; brain; brain ventricle; chemically induced disorder; drug antagonism; fungus; human; hyperlipidemia; intravenous drug administration; learning disorder; male; maze test; memory disorder; metabolism; Monascus; parasitology; pathogenicity; pathology; rat; rice; secretion; Wistar rat; Alzheimer Disease; Amyloid beta-Protein; Amyloid Precursor Protein Secretases; Animals; Brain; Cerebral Ventricles; Cholesterol; Fungi; Humans; Hyperlipidemias; Infusions, Intravenous; Learning Disorders; Lovastatin; Male; Maze Learning; Memory Disorders; Monascus; Oryza sativa; Peptide Fragments; Rats; Rats, Wistar; Reactive Oxygen Species; Thiobarbituric Acid Reactive Substances; Animalia; Rattus
Type
journal article