The effect of β-glucan on the in vitro haemocytes of Litopenaeus vannamei
Date Issued
2014
Date
2014
Author(s)
Leong, Eddie Shu Pu
Abstract
The present study aims to examine the effects of mushroom β-glucan on the cultured Litopenaeus vannamei haemocytes via in-vitro experiment. The cell viability and activity were enhanced, while the osmolarity of cell cultured medium was adjusted into the 900±5mOsm/Kg compared to the unadjusted （315±5mOsm/Kg） cultured medium. Further examination also presented that the adjusted cell cultured medium additional supplemented with the 0.5% β-glucan induce the prolonger of the cell survival compared to the unadjusted group. In the ROS production and apoptosis examination, while the shrimp haemocytes cultured in the adjusted cell cultured medium supplemented with the β-glucan presented that apoptosis cituation of the haemocyte was to be reduced but the ROS production was to be enhanced. The phagocytosis ability, cell particles and sizes of the haemocyte cultured in the adjusted cell culture medium supplemented with β-glucan, we observed that the complication of the intracellular particle and the cell size were to be complicated via the FLOW Cytometry observation and the phagocytosis ablity was to be increased.
In this research, we observed that the osmolarity of the shrimp cultured medium was supposed to be adjusted in to 900 ±5mOsm/Kg to maintain the cell viability and activity compared to the unadjusted culture medium in the lab experiment. And we also presented that the β-glucan exactly induce the survival rate, phagocytosis activity and the ROS production of shrimp haemocytes, but the reduce the apoptosis of the shrimp haemocytes via the in-vitro experiment.
Subjects
南美白蝦
血淋巴細胞
細胞培養
滲透壓
流式細胞儀
菇蕈多醣體
Type
thesis
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