Rapid Detection of Human Immunodeficiency Virus Type 1 Subtype E Infection by Pcr
Resource
JOURNAL OF CLINICAL MICROBIOLOGY v.40 n.10 pp.3805-3809
Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Journal Volume
v.40
Journal Issue
n.10
Pages
3805-3809
Date Issued
2002
Date
2002
Author(s)
CHEN, MAO-YUAN
WANG, WEI-KUNG
TWU, SHIING-JER
WU, SHIOW-ING
LEE, CHUN-NAN
Abstract
The CRF01_AE (subtype E) strain of human immunodeficiency virus type I ( HIV-1), originally reported in Thailand, spread rapidly to and showed prevalence in several countries in Southeast Asia, including Taiwan. This strain was also found in other regions of the world. Based on sequence analysis of the vpu gene, a nested PCR assay including an outer primer pair and a subtype E-specific inner primer pair was developed in this study for rapid detection of subtype E viruses. It was tested with 397 HIV -1-positive samples of known subtypes. For these samples, the sensitivity of detection of subtype E viruses was 100% (127 of 127), and the specificity was 97.8% (264 of 270). Although six samples of either subtype A or G showed a positive PCR, most of the cross-reactivity could be reduced by raising the annealing temperature from 54degreesC to 63degreesC . When tested with 195 HIV-positive samples of unknown subtypes, the assay had a sensitivity of 98.0% and a specificity of 98.6%. This is a simple, convenient, and sensitive method for rapid detection of subtype E viruses, especially in regions in which viruses of subtypes B and E are predominant.
Subjects
LANGERHANS CELL TROPISM
HIV TYPE-1
MOLECULAR EPIDEMIOLOGY
GENETIC DIVERSITY
VIRAL LOAD
TRANSMISSION
SDGs
Type
journal article