Proteomics Laboratory for Cardiovascular Diseases : The Studies of Dialated Cardiomyopathy via Proteomics Approach
Date Issued
2001-07-31
Date
2001-07-31
Author(s)
許榮彬
DOI
892314B002285
Abstract
We employed proteomics methods to analyze myocardial proteins derived from DCM
myocardium, particularly myofibril proteins. Dilated cardiomyopathy (DCM) is a severe heart
disease leading to heart insufficiency and many patients require heart transplantation to correct
the disease. We successfully isolated myofibril proteins using differential extraction method
and then resolved them on a 2D gel electrophoresis system. The identities of proteins are
verified using liquid chromatography-tandem mass spectrometry and specific modifications on
these proteins can also be identified. Using myosin regulatory light chain 2 (mlc-2) as an
example, we demonstrated that how modification is mapped using selected ion tracing
approach. We also employed the similar approach to study proteins involved in energy
metabolism. As an example, the ATP synthase subunit was identified on the 2D gel and
analyzed for its post-translational modifications. Surprisingly, no modification was found
beside the methionine oxidation. We have been investigating the post-translational
modification of other cardiac proteins. These data will be complied to identify myocardiumspecific
modifications as well as to find markers for DCM hearts.
Subjects
dilated cardiomyopathy
proteomics
tandem mass spectrometry
twodimensional
gel electrophoresis
gel electrophoresis
SDGs
Publisher
臺北市：國立臺灣大學醫學院外科
Type
report
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