In vivo multiphoton imaging of obstructive cholestasis in mice
Journal
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Journal Volume
7569
Date Issued
2010
Author(s)
Abstract
Combining multiphoton microscopy with a newly designed hepatic imaging window, we acquired in vivo images of mice obstructive cholestasis. We observed that in mice with bile duct ligation, bile canaliculi failed to appear during the whole observation period over 100 minutes following carboxyfluorescein diacetate injection, whereas the fluorescence was retained much longer within sinusoids. Furthermore, the fluorescence intensities in sinusoids were persistently higher than in hepatocytes during the course.
Type
conference paper