Multi-wavelength quantitative differential phase contrast imaging by radially asymmetric illumination
Journal
Optics Letters
Journal Volume
44
Journal Issue
18
Pages
4542-4545
Date Issued
2019
Author(s)
Abstract
A new approach for achieving isotropic differential phase contrast imaging by applying multi-wavelength asymmetric illumination is demonstrated. Multi-wavelength isotropic differential phase contrast scheme (MW-iDPC) can be implemented using an add-on module in any commercial inverted microscope. Isotropy of intensity transfer function is achieved using three axis measurements. The expression for MW-iDPC imaging is presented, and detailed mathematical analysis is performed for transfer function. By applying color leakage correction, image sensor responses can be calibrated. Asymmetric illumination masks are designed, and simulation studies for intensity of the transfer function are performed. We utilize the MW-iDPC system to reconstruct quantitative phase images of standard microspheres and live breast cancer cells. The optical thickness of cells can be measured with high accuracy and image acquisition time is reduced significantly. ? 2019 Optical Society of America
SDGs
Other Subjects
Medical imaging; Breast cancer cells; Differential phase contrast; Differential phase contrast imaging; Illumination mask; Image acquisition time; Mathematical analysis; Multi-wavelengths; Simulation studies; Transfer functions
Publisher
OSA - The Optical Society
Type
journal article