Tissue-direct PCR, a rapid and extraction-free method for barcoding of ferns.
Resource
Molecular Ecology Resources,10,92-95.
Journal
Molecular Ecology Resources
Journal Issue
10
Pages
92-95
Date Issued
2010
Date
2010
Author(s)
Abstract
Fern gametophytes and young sporophytes often provide too little material for DNA extraction and are particularly difficult to identify to genus. Here we developed an efficient procedure called 'Tissue-direct PCR', in which a slice of fern tissue is mixed with PCR reagents and primers, allowing certain genomic regions to be amplified directly in the thermal cycler. For these diminutive and featureless stages of ferns, Tissue-direct PCR combined with amplifying plant barcodes promises to make the identification of immature ferns easy and rapid. Tissue-direct PCR would also be very helpful for large-scale ecological studies surveying distribution and population structure.
SDGs