A CMOS cantilever-based label-free DNA SoC with improved sensitivity for hepatitis B virus detection
Journal
IEEE Transactions on Biomedical Circuits and Systems
Journal Volume
7
Journal Issue
6
Pages
820-831
Date Issued
2013
Author(s)
Abstract
This paper presents a highly-integrated DNA detection SoC, where several kinds of cantilever DNA sensors, a readout circuit, an MCU, voltage regulators, and a wireless transceiver, are integrated monolithically in a 0.35~μm CMOS Bio-MEMS process. The cantilever-based biosensors with embedded piezoresistors aim to transduce DNA hybridization into resistance variation without cumbersome labeling process. To improve detection sensitivity for low DNA concentration use, an oscillator-based self-calibrated readout circuit with high precision is proposed to convert small resistance variation (0.6~Ω ,< 0.02\% of original resistance) of the sensor into adequate frequency variation and further into digital data. Moreover, its wireless capacity enables isolation of the sample solution from electrical wire lines and facilitates data transmission. To demonstrate the effectiveness of full system, it is applied to detect hepatitis B virus (HBV) DNA. The experimental results show that it has the capability to distinguish between one base-pair (1-bp) mismatch DNAs and match DNAs and achieves a limit of detection (LOD) of less than 1 pM. ? 2013 IEEE.
Subjects
Cantilever; DNA SoC; hepatitis B virus; label-free; oscillator-based
SDGs
Other Subjects
Cantilever; Detection sensitivity; Frequency variation; Hepatitis B virus; Label free; oscillator-based; Resistance variations; Wireless transceiver; Nanocantilevers; Readout systems; Sensitivity analysis; Sensors; Viruses; Voltage regulators; DNA; virus DNA; article; equipment; genetic procedures; genetics; Hepatitis B virus; isolation and purification; limit of detection; microchip analysis; semiconductor; Biosensing Techniques; DNA, Viral; Hepatitis B virus; Limit of Detection; Microchip Analytical Procedures; Molecular Probe Techniques; Semiconductors
Type
journal article