New cytotoxic and anti-inflammatory steroids from the soft coral Klyxum flaccidum
Journal
Bioorganic & Medicinal Chemistry Letters
Journal Volume
26
Journal Issue
14
Pages
3253--3257
Date Issued
2016-07
Author(s)
Abstract
Four new steroids, namely klyflaccisteroids G–J (1–4) were isolated from the Formosan soft coral Klyxum flaccidum. The structures of compounds 1–4 were established by spectral data analysis (IR, MS, 1D and 2D NMR) and comparison of spectral data with those of the related known compounds. Cytotoxicity assay revealed that 4 exhibited inhibition activity against the growth of HT-29, P388 and K562 cancer cell lines, whereas 2 showed selective cytotoxicity toward P388 cells. Compound 4 was also found to display significant anti-inflammatory activity for suppressing superoxide anion generation (O2 ?) and elastase release. ? 2016 Elsevier Ltd
Subjects
Anti-inflammatory activity; Cytotoxicity; Klyxum flaccidum; Soft coral; Steroid
SDGs
Other Subjects
2 morpholino 8 phenylchromone; antiinflammatory agent; cytotoxic agent; doxorubicin; elastase; klyflaccisteroid G; klyflaccisteroid H; klyflaccisteroid I; klyflaccisteroid J; Klyxum flaccidum extract; natural product; steroid; superoxide; unclassified drug; antineoplastic agent; nonsteroid antiinflammatory agent; pancreatic elastase; steroid; superoxide; animal cell; antiinflammatory activity; Article; carbon nuclear magnetic resonance; controlled study; coral; drug cytotoxicity; drug isolation; heteronuclear multiple bond correlation; heteronuclear single quantum coherence; HT 29 cell line; human; human cell; infrared spectroscopy; K562 cell line; Klyxum flaccidum; mass spectrometry; nonhuman; nuclear Overhauser effect; P388 cell line; proton nuclear magnetic resonance; stereochemistry; structure activity relation; animal; antagonists and inhibitors; Anthozoa; cell proliferation; chemical structure; chemistry; dose response; drug effects; drug screening; isolation and purification; metabolism; mouse; tumor cell line; Animals; Anthozoa; Anti-Inflammatory Agents, Non-Steroidal; Antineoplastic Agents; Cell Line, Tumor; Cell Proliferation; Dose-Response Relationship, Drug; Drug Screening Assays, Antitumor; Humans; Mice; Molecular Structure; Pancreatic Elastase; Steroids; Structure-Activity Relationship; Superoxides
Type
journal article