Dynamic observation and quantification of type I/II collagen in chondrogenesis of mesenchymal stem cells by second-order susceptibility microscopy
Journal
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Journal Volume
10881
Date Issued
2019
Author(s)
Lee S.-L., Hsueh C.-M., Huang Y.-D., Lee H.-S., Dong C.-Y.
Hsueh C.-M.
Huang Y.-D.
Abstract
Second order susceptibility microscopy was used to image and characterize chondrogenesis in cultured human mesesnchymal stem cells (hMSCs). Second order susceptibility analysis shows that the second order susceptibility tensor ratios can be used to characterize type I and II collagens in living tissues and that both collagen types are produce at the onset of chondrogenesis. Time-lapse analysis shows that relative to type I collagen, type II collagen increases with time. Eventually, type II collagen content stabilizes at the composition of 70% of total collagen content. This approach can be used to continuously and non-invasively monitor the production of collagens I/II and developed into an effective quality control tool for monitoring extracellular matrix production in engineered tissues.
SDGs
Publisher
SPIE
Type
conference paper