Immunomodulatory Activity of Fermented Milk Containing Lactobacillus casei Strain Shirota in BALB/c Mice
Date Issued
2007
Date
2007
Author(s)
Su, Heng-Kuan
DOI
zh-TW
Abstract
The physiological functions of lactic acid bacteria (LAB) include improving intestinal tract health, reducing symptoms of lactose intolerance, decreasing blood serum cholesterol and enhancing the immune system. The aim of this study is to evaluate the immunomodulatory effects of fermented milk with Lactobacillus casei strain Shirota (LcS) in BALB/c mice. The animal models were used to evaluate for non-specific and specific immune responses on the basis of the regulations of “Health-Promoting Food” of FDA, Taiwan. In this experiment, 60 BALB/c mice were divided into 5 groups according to their weight, and gave them commercial chow diet with supplementary trial fermented milk by oral administration of blank (distilled water), control (the same component medium without fermented), low, medium and high dosages (equivalent to 50, 100, 300 mL/60 kg/day for adult). The results of the non-specific immune study showed that three experimental groups significantly increased phagocytosis and natural killer (NK) cells activities compared with blank group. High dosage group significantly enhanced phagocytosis activities, as well as medium and high dosage groups enhanced NK cells activities compared with control group. These results showed that improved innate immunity. Splenocyte proliferation significantly increased in low dosage group compared with blank group by lipopolysaccharide (LPS) stimulating, and stimulated B cells to activate. The cytokines secretions significantly decreased in interleukin-2 (IL-2), but increased in interferon-γ (IFN-γ), IL-4 and IL-5 at all three dosages compared with blank group. But compared with control group, we observed that three dosages significantly promoted entire secretions of IL-2, IFN-γ, IL-4 and IL-5, and the high dosage group significantly increased immunoglobulin G (IgG) and IgM, and that activated B cells. The results of the specific immune study showed that phagocytosis activity was significantly enhanced compared with blank and control groups. The medium and high groups significantly enhanced NK cells activities compared with blank and control groups, showed that they promoted immunity. Splenocyte proliferation significantly decreased in high dosage compared with blank and control groups by phytohemagglutinin (PHA) stimulating. The cytokines secretions significantly increased in IL-2 and IL-12, and decreased in IL-5 at all three dosages compared with blank group. The low and medium dosage groups significantly increased in serum IgA and IgM compared with control group to promote B cells to activate. The OVA specific antibodies significantly decreased in IgG1 at high dosage group, and significantly increased in IgG2a at low and medium dosage groups to regulate Th1/Th2 balance.
Subjects
免疫調節
代田菌
發酵乳
BALB/c
卵白蛋白
immunomodulation
Lactobacillus casei strain Shirota
fermented milk
ovalbumin
Type
thesis
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