Application of two-photon microscopy to elucidate oleic acid-induced changes in microscale transdermal transport processes
Journal
Proceedings of SPIE - The International Society for Optical Engineering
Journal Volume
4262
Pages
217-230
Date Issued
2001
Author(s)
Abstract
In a novel application of two-photon scanning fluorescence microscopy (TPM), three-dimensional spatial distributions of the hydrophilic and hydrophobic fluorescent probes, sulforhodamine B (SRB) and rhodamine B hexyl ester (RBHE), in excised full-thickness human cadaver skin were visualized and quantified. These findings utilizing TPM demonstrate that, in addition to providing three-dimensional images that clearly delineate probe distributions in the direction of increasing slun depth, the subsequent quantification of these images provides additional important insight into the mechanistic changes in transdermal transport underlying the visualized changes in probe distributions across the slun.
Type
conference paper