Probing invadosomes: technologies for the analysis of invadosomes
Journal
The FEBS journal
Journal Volume
289
Journal Issue
19
Pages
5850 - 5863
Date Issued
2022-10-01
Author(s)
Abstract
Invadosomes are protrusive and mechanosensitive actin devices critical for cell migration, invasion, and extracellular matrix remodeling. The dynamic, proteolytic, and protrusive natures of invadosomes have made these structures fascinating and attracted many scientists to develop new technologies for their analysis. With these exciting methodologies, many biochemical and biophysical properties of invadosomes have been well characterized and appreciated, and those discoveries elegantly explained the biological and pathological effects of invadosomes in human health and diseases. In this review, we focus on these commonly used or newly developed methods for invadosome analysis and effort to reason some discrepancies among those assays. Finally, we explore the opposite regulatory mechanisms among invadosomes and focal adhesions, another actin-rich adhesive structures, and speculate a potential rule for their switch.
Subjects
focal adhesion; invadosome dynamics; proteolytic activity; protrusive force; stiffness
SDGs
Publisher
WILEY
Type
review