Peptides mimicking viral proteins of porcine circovirus type 2 were profiled by the spectrum of mouse anti-PCV2 antibodies
Journal
BMC Immunol.
Journal Volume
18
Journal Issue
25
Pages
18-25
Date Issued
2017
Author(s)
Abstract
Background: Porcine circovirus 2 (PCV2) is a small, non-enveloped DNA virus causing swine lymphocyte depletion and severe impact on the swine industry. The aim of this study was to evaluate the antigenicity and immunogenicity of specific peptides, and seeking the potential candidate of PCV2 peptide-based vaccine. It's initiating from peptides reacting with PCV2-infected pig sera and peptide-immunized mouse sera. Results: The data showed that the sera from PCV2-infected pigs could react with the N-terminal (C1), middle region (C2), and C-terminal peptide (C3) of PCV2 capsid protein (CP), ORF3 protein (N1), ORF6 protein (N2) and ORF9 protein (N3). This study demonstrated that anti-PCV2 mouse antisera could be generated by specific synthetic peptides (C3 and N2) and recognized PCV2 viral protein. We found that the tertiary or linear form C-terminal sequence (C3) of PCV2 capsid peptide only appeared a local distribution in the nucleus of PCV2-infected PK cells, virus-like particles of PCV2 major appeared a local distribution in the cytoplasm, and ORF 6 protein of PCV2 were shown unusually in cytoplasm. Furthermore, most residues of the C1 and the C3 were presented on the surface of PCV2 CP, in the view of 3-D structure of the CP. Our data demonstrated that PCV2-infected pigs had higher OD405 value of anti-C3 IgG on Day 1, Month 3 and Month 6 than in Month 1. These pigs had higher anti-C3 IgM level in Month 3 and Month 6 than on Day 1 (P < 0.01). Conclusions: We demonstrated that the key peptide (C3) mimic the C-terminal of PCV2 capsid protein which were capable of inducing antibodies. The specific antibody against the C3 were confirmed as the serological marker in PCV2-infected pigs. ? 2017 The Author(s).
Subjects
Antibody; Mimic; Open reading frame proteins; Peptide; Porcine circovirus type 2
SDGs
Other Subjects
capsid protein; cysteinylarginylthreonylthreonylvalyllysylthreonylpropylseryltryptophylalanylvalylaspartylmethionylmethionylarginylphenylalanylasparaginylisoleucylasparaginylaspartylphenylalanylleucylpropylpropylglycylglycylglycylserine; cysteinylmethionylalanylserylserylthreonylpropylalanylserylpropylalanylpropylserylaspartylisoleucylleucylserylserylglutamylpropylglutaminylserylglutamylalanylpropylpropylglycylarginyltryptophylthreonine; cysteinylserylpropylisoleucylthreonylglutaminylglycylaspartylarginylglycylvalylglycylserylserylalanylvalylisoleucylleucylaspartylaspartylasparaginylphenylalanylvalylthreonyllysylalanylthreonylalanylleucylthreonine; immunoglobulin G; immunoglobulin M; peptide; unclassified drug; viral protein; virus antibody; antiserum; capsid protein; epitope; peptide; viral protein; virus antibody; virus vaccine; amino terminal sequence; animal cell; animal experiment; Article; carboxy terminal sequence; cell nucleus; controlled study; cytoplasm; female; male; newborn; nonhuman; open reading frame; peptide analysis; pig; Porcine circovirus 2; virus like agent; animal; Bagg albino mouse; cell culture; chemistry; Circoviridae infection; Circovirus; immunology; metabolism; molecular mimicry; mouse; sequence alignment; Animals; Antibodies, Viral; Capsid Proteins; Cells, Cultured; Circoviridae Infections; Circovirus; Female; Immune Sera; Immunodominant Epitopes; Male; Mice; Mice, Inbred BALB C; Molecular Mimicry; Peptides; Sequence Alignment; Swine; Viral Proteins; Viral Vaccines
Type
journal article
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2017_BMC Immunology 18(25)_Peptides mimicking viral proteins of porcine circovirus type 2 were profiled by the spectrum of mouse anti-PCV2 antibodies.pdf
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