Functional Studies of FIN219, COP1 and COI1 Proteins in the Integration of Far-red Light and Jasmonate Signaling in Arabidopsis
Date Issued
2015
Date
2015
Author(s)
Lo, Yi-Ying
Abstract
Photomorphogenic development of plants needs the integration of light and phytohormones. FAR-RED INSENSITIVE 219 (FIN219) interacted with CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) that was transferred from the nucleus to the cytoplasm in the light. Interestingly, FIN219 overexpression results in changes of COP1 subcellular localization from the nucleus to the cytoplasm even in the dark. FIN219/JASMONATE RESISTANT 1 (JAR1) catalyzes the synthesis of JA-isoleucine (JA-Ile), and JA-Ile is received by CORONATINE INSENSITIVE 1 (COI1). However, the molecular mechanism underlying the integration of light and jasmonate signaling pathways remains largely uncertain. Furthermore, COP1 has crosstalk with several phytohormones, but with jasmonate or not is still unknown. Thus, we wonder whether changes of COP1 subcellular locations from the nucleus to the cytoplasm under the dark are caused by FIN219-mediated JA-Ile. We are also interested in the relationship among FIN219, COP1 and COI1 in the integration of far-red light and jasmonate signaling. Here, we found that cop1-4 mutant is more insensitive to methyl-JA (MeJA) than Col-0. COP1 acts as a positive and a negative regulator in MeJA-promoted chlorophyll and anthocyanin accumulation, respectively. In addition, examination of phenotype, transcription and translation levels reveals that COP1 is involved in jasmonate signaling pathway and COI1 affects the protein levels of light signaling components. Furthermore, COP1 can interact with FIN219 under the dark, and this interaction is enhanced by MeJA. Strikingly, MeJA treatment can elevate the level of COP1 accumulation in the cytoplasm under the dark and result in the phenotype similar to the photomorphogenic development of seedlings. Taken together, FIN219, COP1, and COI1 coordinate far-red light and jasmonate signaling pathways, and play important roles in plant growth and development.
Subjects
Light
Jasmonates
COI
Arabidopsis seedling development
Type
thesis
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