Persistence of betanodavirus in Barramundi brain (BB) cell line involves the induction of Interferon response
Resource
Fish & Shellfish Immunology 21 (5): 540-547
Journal
Fish & Shellfish Immunology
Journal Volume
21
Journal Issue
5
Pages
540-547
ISSN
1050-4648
Date Issued
2006-11
Date
2006
Author(s)
Abstract
The BB cell line derived from the brain tissue of a barramundi (Lates calcarifer) that survived nervous necrosis virus (NNV) infection is persistently infected with NNV. To elucidate whether interferon (IFN) plays a role in the mechanism of NNV-persistent infection in BB cell line, a virus-negative control cell line was obtained by treating BB cells with NNV-specific rabbit antiserum for 5 subcultures. After the treatment, NNV titer or RNA or capsid protein was no longer detected in the cured BB (cBB) cells. Expression of Mx gene, encoding a type I IFN-inducible antiviral protein, was found in BB cells and cBB cells following NNV infection, but not in NNV-free cBB cells. Moreover, expression of Mx gene and antiviral activity against NNV were induced in cBB cells by the treatment with MAb-neutralized BB cell supernatant. Furthermore, NNV persistent infection was induced again in cBB cell culture if multiplicity of infection (MOI) was low (< or = 1). These experimental results indicated that IFN-like cytokines existed in the culture supernatant of BB cells, and IFN-induced response played an important role in protecting the majority of cells from virus lytic infection and regulating NNV persistence in the BB cell line.
Subjects
BB cell line
Betanodavirus
Fish nodavirus
Interferon
Nervous necrosis virus
Persistent infection
SDGs
Publisher
Academic Press
Type
journal article
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