Molecular detection of wild and cultured fish infected by betanodavirus and iridovirus in Taiwan
Date Issued
2009
Date
2009
Author(s)
Yang, Yen-Nan
Abstract
Betanodavirus and iridovirus are the major viral pathogens that seriously damage aquaculture industry in Taiwan and other Asian countries. Although virus surveillance has been performing for cultured fish in Taiwan, however, there are few reports of the detection of both viruses in coastal fish. In this study, molecular detection methods such as PT-PCR and PCR were used to detect betanodavirus and iridovirus. To detect trace amount of virus in the healthy carrier fish, the Nested-PCR method was further introduced to increase the detection sensitivity. In total 195 wild fish samples, 50 samples were positive in NNV Nested-PCR (detection rate 25.6%), 32 samples were positive in Ranavirus Nested-PCR (detection rate 16.4%), and no sample was positive in Megalocytivirus Nested-PCR. These data indicate that wild fish were NNV and Ranavirus carriers around Taiwan seashore in general. Seven fish (6 from Yi-Lan, 1 from Keenting) were positive both in NNV and Ranavirus Nested-PCR. Therefore, it is possible that one individual fish can be simultaneously infected by two viruses in nature. The phylogenic tree shows that 34 of 38 NNV samples belong to RGNNV group (89.4%), whereas 4 belong to SJNNV group (10%). All 33 of Ranavirus samples belong to GIV and SGIV group, but not BIV (Bohle iridovirus) nor EHNV (Epizootic haematopoietic necrosis virus) group. Both phylogenic tree and sequence similarity of Megalocytivirus (CY15 amplicon gene) from Peng-Hu cultured Tiger grouper (Epinephelus fuscoguttatus) are closer to LYCIV (Large Yellow Croaker Iridovirus) in Fujian but not to TGIV(Grouper Iridovirus of Taiwan).
Subjects
detection
nodavirus
iridivirus
Megalocytivirus
NNV
GIV
Ranavirus
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