Chemical constituents from a red algae-associated fungus Trichoderma harzianum NTOU4253 isolated from Taiwan
Date Issued
2016
Date
2016
Author(s)
Chang, Wen-Husan
Abstract
Bioassay-guided fractionation and separation of the active component from Trichoderma harzianum NTOU4253 resulted in the isolation of six compounds 1-6. Their structurues were determined to be linear C13 polyketides, and four new compounds were named trichoharol A (1), trichoharol B (4), trihcoharol C (5) and (−)-harzianolide (6), together with previously reported two polyketide-type C13 lipids harzianumols A (2) and harzianumols B (3). Compound 2, 4 and 5 exhibited moderate to potent inhibitory activity on NO production of RAW264.7 cells induced by lipopolysaccharide (LPS) with IC50 values of 31.1, 51.3 and 19.7 μM, respectively, without any cytotoxicity. The IC50 values of the positive controls, aminoguanidine and Nω-nitro-L-arginine (L-NNA), were 22.3 ± 0.3 and 146.4 ± 0.7 μM, respectively. Compound 5 exhibited slight antimicrobial activities against Staphylococcus aureus with 15.0 ± 0.3 mm inhibition zone compared to positive control-tetracycline with inhibition zone of 25.0 ± 0.1 mm.
Subjects
fungi
Hypocreaceae
nitric oxide
polyketide
trichoharol
antimicrobial activity
Type
thesis
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