ynthesis of inorganic nanoparticles/organic alginate composite for guiding chemotherapy of bladder cancer
Date Issued
2014
Date
2014
Author(s)
Wang, Yu-Pu
Abstract
The purpose of this study is to fabricate a composite consisting of organic alginate and inorganic nanoparticles (including hydroxyapatite, Fe3O4, CaBO3, and ZIF-8) for biomedical applications. There are two types of composites synthesized in this study: (1) alginate-based microspheres and (2) alginate-based nanoparticles. For the part of alginated-based microspheres, proteins and hydroxyapatite nanoparticles can be encapsulated into alginate microspheres with diameters around 20-30 μm (named as LSZ-HA@Alg) via air dynamical atomization method. For the part of alginated-based nanoparicles, inorganic nanoparticles such as Fe3O4 and CaP were synthesized within the organic alginate polymer through a combination of pre-gel method and co-precipitation. We then functionalize Fe3O4@CaP-Alg nanocomposite with c(RGDfK) peptide for targeting bladder cancer cell line (i.e. T24). Fe3O4@CaP-Alg nanocomposite exhibits superparamagnetic property of Fe3O4, and the CaP shell can encapsulate an antitumor drug, doxorubicin with an encapsulation efficiency of about 80 %. With ICP-OES measurements, the accumulation of Fe3O4 in T24 for Fe3O4@CaP-Alg-RGD is 3.13 times more than that of Fe3O4@CaP-Alg, indicating the cell targeting ability. Furthermore, the relative cell viabilities decrease to 0.1-0.3, depending on the concentration of Fe3O4@CaP(+)-Alg-RGD. We can use magnetism to guide the synthesized nanocomposite to specific locations that can increase the local concentration of material and result in better inhibition of the cancer cell with the lowest impact on adjacent cells, which would be useful for guiding chemotherapy of bladder cancer.
Subjects
空氣動力噴霧法
預凝膠
海藻酸
誘導化學治療
膀胱癌
SDGs
Type
thesis
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