The potential of BMVC in early detecting the pre-malignant transform of cancer
Date Issued
2011
Date
2011
Author(s)
Lin, Lin
Abstract
Advanced diagnostic tools stand today at the heart of successful cancer treatment. BMVC is a new immunofluorescence staining technology that the distinct fluorescence expression properties can be used to distinguish cancer cells from normal cells. Using this technology, normal cells showed negative BMVC staining while the bright fluorescence spots from BMVC in neoplastic cells could be observed. In the present study, we go further steps to evaluate whether BMVC analysis could be used in the detection of pre-malignant neoplasm. Twenty different stages of morphologically transformed BALB/3T3 cells were induced by the carcinogenic agents 3-methylcholanthrene(MCA). These clonal populations were spotlighted on the expression of selected transformation phenotypes, including loss of contact inhibition, enhance migratory ability, hyperproliferative, evasion of programmed cell death, anchorage-independent and invade into extracellular matrices in vitro. The results suggested that the difference of these transformation phenotypes are correlated with the expression of BMVC, particularly in anchorage independence. We have demonstrated that BMVC can be deployed to distinguish normal cells from transforming cells. Most significantly, BMVC can detect cells in their early stages of cancerous transformed cells. The study shows the potential of BMVC in early detection of transformation events.
Subjects
cancer
early detection
pre-malignant
contact inhibition
transformation
SDGs
Type
thesis
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