Anti?inflammatory fibronectin?agnp for regulation of biological performance and endothelial differentiation ability of mesenchymal stem cells
Journal
International Journal of Molecular Sciences
Journal Volume
22
Journal Issue
17
Date Issued
2021
Author(s)
Abstract
The engineering of vascular regeneration still involves barriers that need to be conquered. In the current study, a novel nanocomposite comprising of fibronectin (denoted as FN) and a small amount of silver nanoparticles (AgNP, ~15.1, ~30.2 or ~75.5 ppm) was developed and its biological function and biocompatibility in Wharton’s jelly?derived mesenchymal stem cells (MSCs) and rat models was investigated. The surface morphology as well as chemical composition for pure FN and the FN?AgNP nanocomposites incorporating various amounts of AgNP were firstly characterized by atomic force microscopy (AFM), UV?Visible spectroscopy (UV?Vis), and Fourier?transform infrared spectroscopy (FTIR). Among the nanocomposites, FN?AgNP with 30.2 ppm silver nanoparticles demonstrated the best biocompatibility as assessed through intracellular ROS production, proliferation of MSCs, and monocytes activation. The expression levels of pro?inflammatory cytokines, TNF?α, IL?1β, and IL?6, were also examined. FN?AgNP 30.2 ppm significantly inhibited pro?inflammatory cytokine expression compared to other materials, indicating superior performance of anti?immune response. Mechanistically, FN?AgNP 30.2 ppm significantly induced greater expression of vascular endothelial growth factor (VEGF) and stromal?cell derived factor?1 alpha (SDF?1α) and promoted the migration of MSCs through matrix metalloproteinase (MMP) signaling pathway. Besides, in vitro and in vivo studies indicated that FN?AgNP 30.2 ppm stimulated greater protein expressions of CD31 and von Willebrand Factor (vWF) as well as facilitated better endothelialization capacity than other materials. Furthermore, the histological tissue examination revealed the lowest capsule formation and collagen deposition in rat subcutaneous implantation of FN? AgNP 30.2 ppm. In conclusion, FN?AgNP nanocomposites may facilitate the migration and proliferation of MSCs, induce endothelial cell differentiation, and attenuate immune response. These finding also suggests that FN?AgNP may be a potential anti?inflammatory surface modification strategy for vascular biomaterials. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Subjects
Endothelial differentiation
Fibronectin
Mesenchymal stem cells
Silver nanoparticles
Vascular tissue engineering
antiinflammatory agent
fibronectin
matrix metalloproteinase
metal nanoparticle
reactive oxygen metabolite
silver
animal
cell culture
cell differentiation
cell proliferation
cytology
cytoskeleton
drug effect
endothelium cell
immunohistochemistry
infrared spectroscopy
mesenchymal stem cell
metabolism
particle size
rat
ultrastructure
Animals
Anti-Inflammatory Agents
Cell Differentiation
Cell Proliferation
Cells, Cultured
Cytoskeleton
Endothelial Cells
Fibronectins
Immunohistochemistry
Matrix Metalloproteinases
Mesenchymal Stem Cells
Metal Nanoparticles
Particle Size
Rats
Reactive Oxygen Species
Silver
Spectroscopy, Fourier Transform Infrared
SDGs
Type
journal article