Clitocybe nuda activates dendritic cells and acts as A DNA vaccine adjuvant
Journal
Evidence-based Complementary and Alternative Medicine
Journal Volume
2013
Date Issued
2013
Author(s)
Chen, M.-H.
Li, W.-S.
Lue, Y.-S.
Pan, I.-H.
Ko, C.-H.
Chen, D.-Y.
Lin, C.-H.
Lin, S.-H.
Chang, C.-P.
Lin, C.-C.
Abstract
This work represents the first evaluation of the effects of water extract of C. nuda (WE-CN), an edible mushroom, on murine bone marrow-derived dendritic cells (BMDCs) and the potential pathway through which the effects are mediated. Our experimental results show that WE-CN could induce phenotypic maturation of DCs, as shown by the increased expression of MHC and costimulatory molecules. In addition, it also induced the proinflammatory cytokines expression on DCs and enhanced both the proliferation and IFN-γ secretion of allogenic T cells. Therefore, since WE-CN did not induce maturation of DCs generated from mice with mutated TLR-4 or TLR-2, suggesting that TLR4 and TLR2 might function as membrane receptors for WE-CN. Moreover, the mechanism of action of WE-CN may be mediated by increased phosphorylation of ERK, p38, and JNK mitogen-activated protein kinase (MAPK) and increased NF-B p65 activity, which are important signaling molecules downstream of TLR-4 and TLR-2. Finally, coimmunization of mice with WE-CN and a HER-2/neu DNA vaccine induced a HER-2/neu-specific Th1 response that resulted in significant inhibition of HER-2/neu overexpressing mouse bladder tumor (MBT-2) growth. These data suggest that WE-CN induces DC maturation through TLR-4 and/or TLR-2 and that WE-CN can be used as an adjuvant in cancer vaccine immunotherapy. ? 2013 Mei-Hsing Chen et al.
Other Subjects
Clitocybe nuda water extract; DNA vaccine; epidermal growth factor receptor 2; gamma interferon; major histocompatibility antigen; mitogen activated protein kinase; mitogen activated protein kinase p38; plant extract; stress activated protein kinase; toll like receptor 2; toll like receptor 4; transcription factor RelA; unclassified drug; water; animal cell; animal experiment; animal model; antigen expression; antineoplastic activity; article; bone marrow cell; cell maturation; Clitocybe nuda; controlled study; cytokine release; dendritic cell; drug mechanism; edible mushroom; enzyme activity; female; immunization; lymphocyte proliferation; mouse; nonhuman; priority journal; protein phosphorylation; signal transduction; T lymphocyte
Type
journal article