Isolation and Characterization of a Lectin from Edible Mushroom, Volvariella volvacea
Resource
J. Biochem 96 (1): 35-40
Journal
J. Biochem
Journal Volume
96
Journal Issue
1
Pages
35-40
Date Issued
1984
Date
1984
Author(s)
LIN, Jung-Yaw
Abstract
A lectin was purified from edible mushroom, Volvariella volvacea by extraction with 5% cold acetic acid in the presence of 0.1% 2-mercaptoethanol, followed by ammonium sulfate fractionation, and DEAE-C-52 and CM-C-52 column chromatographies. The molecular weight was measured to be 26,000, and the lectin consisted of two non-identical subunits as demonstrated by gel filtration and polyacrylamide gel electrophoresis. The lectin does not contain half-cystine, methionine, or histidine. The LD50 of the lectin is 17.5 mg per kg body weight of mice. The lectin has a moderate inhibitory effect on the growth of tumor cells.
Type
journal article
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