Assessment of heavy metal bioavailability in contaminated sediments and soils using green fluorescent protein-based bacterial biosensors
Resource
Environmental Pollution 142 (2006) 17e23
Journal
Environmental Pollution
Journal Volume
142
Journal Issue
2006
Pages
-
Date Issued
2006-09-29
Date
2006-09-29
Author(s)
Liao, Hsiu-Chuan
Chien, Ming-Te
Tseng, Yuen-Yi
Ou, Kun-Lin
DOI
246246/2006111501254683
Abstract
A green fluorescent protein (GFP)-based bacterial biosensor Escherichia coli DH5a (pVLCD1) was developed based on the expression of gfp
under the control of the cad promoter and the cadC gene of Staphylococcus aureus plasmid pI258. DH5a (pVLCD1) mainly responded to Cd(II),
Pb(II), and Sb(III), the lowest detectable concentrations being 0.1 nmol L1, 10 nmolL1, and 0.1 nmol L1, respectively, with 2 h exposure.
The biosensor was field-tested to measure the relative bioavailability of the heavy metals in contaminated sediments and soil samples. The
results showed that the majority of heavy metals remained adsorbed to soil particles: Cd(II)/Pb(II) was only partially available to the biosensor
in soilewater extracts. Our results demonstrate that the GFP-based bacterial biosensor is useful and applicable in determining the bioavailability
of heavy metals with high sensitivity in contaminated sediment and soil samples and suggests a potential for its inexpensive application in
environmentally relevant sample tests.
Subjects
bandpass filter
diplexer
microstrip filter
stepped-impedance resonator (SIR)
transmission zero
Green fluorescent protein
Bacterial biosensor
Bioavailable heavy metals
Contaminated sediments and soils
Publisher
Taipei:National Taiwan University
Type
journal article
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