Studies on anticancer activity of ganoderic acid T
Date Issued
2008
Date
2008
Author(s)
Lee, I-Lin
Abstract
Ganoderic acid T (GAT), is a lanostanoid triterpene isolated from Ganoderma lucidum (Leyss.exFr.) Karst., and the aim of this study was to study the effects of GAT on some cancer cell lines. GAT was found to inhibit the growths of human leukemia cell line, HL-60 and hepatoma cell lines, HepG2 and Huh7, and the IC50 are 35.2, 20.3 and 75.7 μM respectively, while GAT showed no effect on non-proliferating human peripheral blood mononuclear cells. In HL-60 cells, Liu’s stain, TUNEL assay, MMP, cell cycle, 7-AAD, caspase-8, caspase-9 were performed. Apoptotic bodies were shown by Liu’s stain, TUNEL assay positive cells were observed in TUNEL assay, and 7-AAD positive cells were observed. Mitochrondrial membrane potential was decreased, and caspase-9 and 3 activities were increased in GAT treatment. In conclusion, GAT was found to induce HL-60 cell apoptosis. In Huh 7 cells, GAT was found to decrease mitochondrial membrane potential and induce cell cycle arrest in G2 phase. In HepG2 cells, preliminary data showed that GAT seemed to induce premature senescence of HepG2 cell. GAT was recovered from cultured medium, and HPLC and Q-TOF mass spectrometry was used to analyze GAT and the recovered fraction. The result showed that GAT is stable in cell culture. In the future, GAT might be recovered from culture medium. The results above indicate that GAT could be a potential anticancer drug.
Subjects
Ganoderma
Ganoderic acid
apoptosis
cell cycle arrest
senescence
SDGs
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