Minor histocompatibility antigen HA-1 and HA-2 polymorphisms in Taiwan: Frequency and application in hematopoietic stem cell transplantation
Journal
Clinical Chemistry and Laboratory Medicine
Journal Volume
48
Journal Issue
9
Pages
1287-1293
Date Issued
2010
Author(s)
Abstract
Background: Minor histocompatibility antigens influence the occurrence of graft-vs.-host disease and graft-vs.-leukemia effects after hematopoietic stem cell transplantation (HSCT). We determined the population frequencies of HA-1 and HA-2 alleles in Taiwan and exploited their potential applications in allogeneic HSCT. Methods: HA-1 and HA-2 were genotyped using polymerase chain reaction and restriction fragment length polymorphism in healthy controls (221 for HA-1 and 306 for HA-2) and HLA-matched donor-recipient sibling pairs with HSCT (92 for HA-1 and 38 for HA-2). The association of genetic polymorphisms with HSCT outcome was evaluated by univariate and multivariate analyses. Results: The allele frequencies in controls were 35.3% and 64.7% for HA-1 H and HA-1R, and 89.0% and 11.0% for HA-2V and HA-2M, respectively. HA-1 disparity was denoted in 16.3% of HLA-matched donor-recipient sibling pairs, while it was not associated with HSCT outcome. HA-2 disparity was not observed in the donor-recipient pairs studied. The possibilities of using HA-1 and HA-2 variabilities as molecular markers for hematopoietic chimerism after HSCT were 39.2% and 18.4%, respectively. Conclusions: Our data provide the information on allele and genotype frequencies of HA-1 and HA-2 in a Taiwanese population, and suggest that prospective genomic typing for HA-1 and HA-2 alleles of the donor and recipient could be a useful approach for molecular identification of hematopoietic chimerism after HSCT, rather than prognosis of clinical outcome. ? 2010 by Walter de Gruyter Berlin New York.
SDGs
Other Subjects
cyclosporin A; HLA antigen; methotrexate; minor histocompatibility antigen; minor histocompatibility antigen HA 1; minor histocompatibility antigen HA 2; molecular marker; thymocyte antibody; unclassified drug; acute graft versus host disease; acute granulocytic leukemia; acute lymphoblastic leukemia; adolescent; adult; aged; allogeneic hematopoietic stem cell transplantation; article; cancer radiotherapy; child; chimera; chronic graft versus host disease; chronic myeloid leukemia; controlled study; female; gene frequency; genetic polymorphism; genotype; graft failure; graft recipient; graft versus host reaction; hematopoiesis; HLA matching; human; infant; leukemia relapse; major clinical study; male; organ donor; outcome assessment; polymerase chain reaction; preschool child; priority journal; restriction fragment length polymorphism; school child; sibling; Taiwan; treatment outcome; Adolescent; Adult; Aged; Asian Continental Ancestry Group; Case-Control Studies; Child; Child, Preschool; Female; Gene Frequency; Genotype; Graft Rejection; Hematopoietic Stem Cell Transplantation; Humans; Infant; Male; Middle Aged; Minor Histocompatibility Antigens; Multivariate Analysis; Polymorphism, Genetic; Recurrence; Siblings; Taiwan; Tissue Donors; Treatment Outcome; Young Adult
Type
journal article