氧化性低密度脂蛋白之結構及其臨床應用研究(2/3)
Date Issued
2005
Date
2005
Author(s)
李源德
DOI
932314B002031
Abstract
The objective of this study was to study the protein profiles of HUVEC regulated
by oxLDL and LDL circulating subfraction by proteomic approaches. In addition, the
candidate proteins which were regulated by oxidized LDL and LDL subfractions were
identified. Human umbilical vein endothelial cells (HUVECs) were incubated with
serum-free medium, native LDL (100 ug/mL), oxLDL (2hr- and 24 hr-oxidized; 100
ug/mL), LDL circulating subfraction (L1, L3 and L5; 50 ug/mL) for 24 hrs, respectively.
MCP-1 was measured as an indicator of inflammation. Cytosolic proteins were applied
in 2D electrophoresis and identified by MALDI-TOF mass-spectrometer. No differences of cell viability and MCP-1 production were observed on dose responses of
24hr- oxLDL treatment. Various grades of copper oxidation of LDL had the same
effects on MCP-1production and cell viability. Circulating subfraction L5 had a highest
inflammatory reaction and caused lower cell viability, suggested that L5 had a higher
atherogenic capability than L1 and L3 did. The protein profiles of oxLDL and LDL
subfraction were different in the preliminary test. However, more replications and
statistical analysis are required to make the conclusion. For those proteins had been
identified at present, most of them were redox-related proteins, peroxiredoxins and heat
shock proteins were the majority.
Publisher
臺北市：國立臺灣大學醫學院內科
Type
report
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