Regulation of EGCG on OSM-stimulated cyr61 expression in human osteoblast
Date Issued
2006
Date
2006
Author(s)
Lan, Yi-Lun
DOI
en-US
Abstract
Cyr61 is a novel protein which is found in recent years, it is well known as the function with cell proliferation, migration, adhesion, formation of extracellular matrix (ECM), angiogenesis and tumorigenesis. However, the role of cyr61 and the regulation mechanism are still unclear now.
In our study, we found that treat osteoblast cells with OSM will up-regulates the expression of cyr61. The results of our experiments reveal that OSM-stimulated cyr61 expression is regulated at transcriptional level, which means mRNA level, but not regulated at post-translational level.
Cyr61 expression may be regulated by Akt and Mitogen-activated protein kinase (MAPK) pathway. We determined that OSM induces cyr61 expression by phosphorylation and activation of Akt (p-Akt) and IkB (p-IkB). Thereafter, activated form of IkB leads to the activation of Nuclear factor-kB (NF-kB) which translocates into the nucleus to regulates gene expression at tran-scriptional level and enhances cyr61 protein expression. Also, in our study, the expression of cyr61 may be regulated by p38 and JNK.
In this study, we also found that in osteoblast cells, EGCG effectively inhibit the activation of Akt, IkB, NF-kB and the expression of cyr61 which are stimulated by OSM.
Subjects
成骨細胞
兒茶素
OSM
cyr61
EGCG
Akt
NF-kB
Type
other
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