Visfatin/PBEF mRNA Expression in Human Adipose Tissue:The Association with Metabolic Phenotypes And Its Regulatory Mechanism
Date Issued
2006
Date
2006
Author(s)
DOI
zh-TW
Abstract
Context: Visfatin/PBEF was recently identified as an adipocytokine predominantly expressed and secreted in visceral adipose tissue with insulin-mimetic effect in peripheral tissues.
Objectives: The goals were to investigate the association of visfatin/PBEF gene expresion in human abdominal adipose tissue with metabolic phenotypes and to identify the
regulatory mechanism of visfatin/PBEF expression in cultured cells.
Subjects and Methods: Relative visfatin/PBEF mRNA levels in human abdominal visceral and subcutaneous adipose tissues were determined by quantitative real-time polymerase chain reaction for correlation with metabolic phenotypes. Regulation of visfatin/PBEF expression by humoral factors was examined using cultured murine3T3-L1 adipocytes and RAW 264.7 macrophages.
Results: Relative visfatin/PBEF mRNA levels in abdominal subcutaneous adipose tissue were higher than those in visceral adipose tissue and neither correlated with BMI.
Relative visfatin/PBEF mRNA levels in abdominal subcutaneous adipose tissue correlated positively with fasting plasma insulin concentrations and with the insulin resistance index by Homeostasis Model Assessment(HOMA-IR). Free fatty acids (palmitate and oleate) and lipopolysaccharide stimulated visfatin/PBEF expression in RAW 264.7 macrophages but not 3T3-L1 adipocytes; the stimulation was mediated by NF-κB activation. Treatment with aspirin, sodium salicylate, or 15-deoxy-Δ12,14-prostaglandin J2 (15-d-PGJ2), but not indomethacin, ketorolac or rosiglitazone, suppressed visfatin/PBEF expression in RAW 264.7 macrophages.
Conclusions: Visfatin/PBEF mRNA expression in abdominal subcutaneous adipose tissue is increased in hyperinsulinemic and insulin-resistant subjects. Visfatin/PBEF expression is induced by free fatty acids and inflammatory stimuli in macrophages rather than adipocytes. These findings support linkages among insulin resistance, macrophage activation, and increased visfatin/PBEF expression.
Subjects
visfatin
PBEF
脂肪組織
巨噬細胞
游離脂肪酸
發炎
adipose tissue
macrophage
free fatty acid
inflammation
Type
text
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