Assessment of gastric mucosal lesion preventive effect for lactic acid bacteria fermented soy-skim milk
Date Issued
2008
Date
2008
Author(s)
Hu, Chun-Ling
Abstract
Lactic acid bacteria (LAB) are reported to improve gastrointestinal health. Furthermore, some reports indicate LAB and their fermented products can effectively enhance the gastric mucosal integrity. The objective of this research was to evaluate the effect of fermented soy-skim milk of LAB on mucosal integrity in gastric mucosal lesion rat. This study used Lactobacillus paracasei subsp. paracasei NTU 101 and Lactobacillus plantarum NTU 102 as a starter to discuss the optimal mix ratio of soymilk and milk. It showed that the cell numbers of L. paracasei subsp. paracasei NTU 101 and L. plantarum NTU 102 fermented in 75% soymilk and 25% milk (optimal condition) after 24-hr cultivation were achieved 1.2×109 CFU/mL and 2.5×109 CFU/mL, respectively. After the shelf-life (180 day, 4oC), the cell numbers of freeze-dried powders from L. paracasei subsp. paracasei NTU 101 and L. plantarum NTU 102 fermented soy-skim milk were maintained at 1×109 CFU/g; variations in pH and titratable acidity were slight. Pylorus ligation combined acidified ethanol was not only able to induce acute gastric lesions, but also get more stable data with collection of gastric juice, experimental operation and parameters of gastric lesion than others in this study. Pylorus ligation combined acidified ethanol treatment was used as animal model of gastric lesion. Experiment groups were fed with RO water (as control), commercial yogurt, unfermented soy-skim milk, L. paracasei subsp. paracasei NTU 101 and L. plantarum NTU 102 fermented soy-skim milk for 28 days, respectively and the preventive effects in the rat of gastric lesion were evaluated. LAB fermented soy-skim milks were resulted in reducing gastric lesion index, gastric lipid peroxides and serum lipid peroxides. They also enhanced the activity of superoxide dismutase (SOD) and cytoprotective prostaglandin E2 (PGE2) synthesis. This finding may indicate that LAB fermented soy-skim milks (109 CFU/day) are the potential food for the protection of gastric injury.
Subjects
fermented soy-skim milk
acute gastric lesion
pylorus ligation
Type
thesis
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