Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity
Journal
Nature Communications
Journal Volume
11
Journal Issue
1
Date Issued
2020
Author(s)
Abstract
Pattern-triggered immunity and effector-triggered immunity are two primary forms of innate immunity in land plants. The molecular components and connecting nodes of pattern-triggered immunity and effector-triggered immunity are not fully understood. Here, we report that the Arabidopsis calcium-dependent protein kinase CPK3 is a key regulator of both pattern-triggered immunity and effector-triggered immunity. In vitro and in vivo phosphorylation assays, coupled with genetic and cell biology-based analyses, show that actin-depolymerization factor 4 (ADF4) is a physiological substrate of CPK3, and that phosphorylation of ADF4 by CPK3 governs actin cytoskeletal organization associated with pattern-triggered immunity. CPK3 regulates stomatal closure induced by flg22 and is required for resistance to Pst DC3000. Our data further demonstrates that CPK3 is required for resistance to Pst DC3000 carrying the effector AvrPphB. These results suggest that CPK3 is a missing link between cytoskeleton organization, pattern-triggered immunity and effector-triggered immunity. © 2020, The Author(s).
Other Subjects
actin; actin 7; actin depolymerizing factor; actin depolymerizing factor 4; alkaline phosphatase; calcium dependent protein kinase 3; F actin; G actin; messenger RNA; protein kinase; reactive oxygen metabolite; unclassified drug; actin depolymerizing factor; ADF4 protein, Arabidopsis; Arabidopsis protein; calcium calmodulin dependent protein kinase; CPK3 protein, Arabidopsis; angiosperm; cell; genetic analysis; immunity; physiological response; protein; skeleton; actin filament; actin polymerization; Agrobacterium tumefaciens; Arabidopsis thaliana; Article; bacterial growth; controlled study; cytology; Escherichia coli; gene interaction; guard cell; immunity; immunoprecipitation; in vitro study; in vivo study; mass spectrometry; Nicotiana benthamiana; nonhuman; protein phosphorylation; protoplast; signal transduction; transient expression; two dimensional electrophoresis; actin filament; amino acid sequence; Arabidopsis; confocal microscopy; genetics; metabolism; microbiology; mutation; physiology; plant disease; plant immunity; Pseudomonas syringae; sequence homology; transgenic plant; Arabidopsis; Embryophyta; Actin Cytoskeleton; Actin Depolymerizing Factors; Amino Acid Sequence; Arabidopsis; Arabidopsis Proteins; Calcium-Calmodulin-Dependent Protein Kinases; Microscopy, Confocal; Mutation; Plant Diseases; Plant Immunity; Plants, Genetically Modified; Pseudomonas syringae; Sequence Homology, Amino Acid
Type
journal article