Characterization of catharanthus roseus genes regulated differentially by peanut witches' broom phytoplasma infection
Journal
Journal of Phytopathology
Journal Volume
159
Journal Issue
7-8
Pages
505-510
Date Issued
2011
Author(s)
Chen, W.-Y.
CHAN-PIN LIN
Abstract
The differential display (DD) strategy was applied to isolate periwinkle (Catharanthus roseus) cDNAs that were differentially expressed following infection with peanut witches' broom (PnWB) phytoplasma. Sixty-four clones were selected from differentially expressed cDNA fragments. Following screening by reverse Northern hybridization, ten transcripts were selected and sequenced. The expression level of each transcript was quantified by real-time PCR, and seven DD transcripts were identified as truly differentially expressed following PnWB phytoplasma infection. Among these, one that was homologous with phi-1 gene was up-regulated, while the others were down-regulated. Except two genes, other four down-regulated genes shared homology with the genes encoding psaDa gene, ML domain protein gene, eukaryotic translation initiation factor SUI1 gene and plastidic aldolase NPALDP1 gene, respectively. The identities of homologous genes were further confirmed for three DD transcripts by isolating long cDNA fragments from the cDNA library that was established in this investigation. Verified genes were ML domain protein gene, translation initiation factor SUI1 gene and plastidic aldolase gene and were primarily involved in the innate immune response, the stress response and photosynthesis. The possible role of these genes in the periwinkle that was infected by PnWB phytoplasma is discussed. ? 2011 Blackwell Verlag GmbH.
Subjects
Differential display; Interaction; Peanut witches' broom phytoplasma; Periwinkle
SDGs
Other Subjects
eukaryote; fragmentation; gene expression; genetic analysis; homology; hybridization; identification method; immune response; infectious disease; legume; perennial plant; photosynthesis; phytoplasma; plastid; polymerase chain reaction; protein; Arachis hypogaea; Candidatus Phytoplasma; Catharanthus roseus; Eukaryota; Peanut witches'-broom phytoplasma
Type
journal article