The Preparation of Cationized Gelatin/Calcium Phosphate Nanoparticles as Gene Carrier for Transgenic Chicken
Date Issued
2009
Date
2009
Author(s)
Huang, Jian-Yuan
Abstract
Due to the hereditary disease, some specific proteins cannot be synthesized for the maintenance of physiological function. The pharmaceutical proteins, prepared in recombinant bacteria, play an important role in the medical engineering. However, most of pharmaceutical proteins are expensive and the production rate is low. Additionally, the proteins prepared from bacteria are inactive or unstable in vivo since they cannot be matured by post-translational modifications. Therefore, the transgenic animal system is a promising bioreactor to produce active proteins having an appropriate biochemical structure. he advantages of using transgenic chickens as bioreactors are the short incubation time, the high production rate, the sterilized contents of the egg, and the stable proteins in egg. Moreover, the contents of the proteins extracted from the hen’s egg compared with the milk from mammals are simpler. Therefore, the transgenic chickens have the potential to be an ideal bioreactor for the production of specific proteins in the future.n this study, the gelatin/calcium phosphate nanoparticles after the cationization can be used as gene carriers applied in the transgenic chickens. Because the particle size is smaller than 1um, it has more possibility for the cell uptake. Using electrophoresis enables to evaluate the DNA loading efficiency, and the result shows that the nanoparticles have ability to carry DNA and protect the DNA as well. The nanoparticles also have higher biocompatibility than the commercial product, LipofecamineTM2000. The transfection efficiency of the cationizated gelatin/CaP nanoparticles achieves at least 40%. In the animal study, it is found that the embryo emits green fluorescence protein observed by the fluorescence microscope after the fourth day of the experiment, so the cationizated gelatin/CaP nanoparticles have potential applied in transgenic chicken.
Subjects
gene carrier
transgenic chicken
gelatin
calcium phosphate
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-98-R96548015-1.pdf
Size
23.53 KB
Format
Adobe PDF
Checksum
(MD5):b211aa79bc86c37929ae05d09f070de3