Modulation of L929 cell adhesion by PEGylated polyelectrolyte multilayer films
Date Issued
2007
Date
2007
Author(s)
Chen, Ying-Hao
DOI
zh-TW
Abstract
Layer-by-layer (LBL) deposition of polyelectrolyte multilayer films is an extremely versatile and useful technique for surface modification of medical devices. Several parameters in preparation of polyelectrolyte multilayer films, such as the number of layers and assembling conditions, might influence the interactions between surface and biological system. In order to reduce cell adhesion on medical devices which may cause undesired clinical complications, polyethylene glycol (PEG) has been considered a non-adhesive biomaterial because it possesses hydrophilic and heavily hydrated character to resist protein adsorption and cell adhesion. The aim of this work was to combine these two advantages, LBL and PEG, together to built up anti-adhesive polyelectrolyte multilayer films (PEMs) to cover the surface with PEG. The L929 cell was seeded onto peglyated PEMs, and cell adhesion, morphology and growth was studied.
We established a layer-by-layer polyelectrolyte multilayer membrane based on poly(ethylene imine) /poly(acrylic acid)(PEI/PAA) with PEG-g-PEI as the topmost layers. Different graft ratios of PEG-g-PEI were synthesized to be different topmost layers to evaluate the efficacy of PEMs on inhibiting L929 cell adhesion and spreading by observing the morphology of L929 cell on the surface. We found that the top layer of pegylated PEI decreased cell adhesion, and L929 cell adhesion and morphology had great difference on the surface with high PEG graft ratios compared to the original TCPS substrate. High graft ratio of PEG-g-PEI increased cell adhesion amounts slightly with increasing number of layers, however cell morphology was still round shape. The high grafted ratio of peglyated PEMs immersed in PBS could stable during two weeks. The cell adhesion on peglyated PEMs of immersed PBS had better inhibition than PEMs without immersed PBS.
Subjects
聚電解質多層膜
聚乙二醇
聚乙烯亞胺
polyelectrolyte multilayer films
polyethylene glycol
polyethylene imine
Type
thesis
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